Capillary Electrophoresis in Quality Control: PART II: CE-SDS: Method Development and Robustness

IB-16385A Abstract Capillary electrophoresis sodium dodecyl sulfate (CE-SDS), is the modern equivalent of the slabgel sizing technique SDS-PAGE. Common uses of SDS-PAGE include monitoring of manufacturing consistency and apparent molecular weight. Although used frequently, SDS-PAGE is a poor technique for quantitative protein purity due to inherent sample preparation artifacts, migration time and staining variability. With greater reproducibility and online detection capability, CE-SDS has not only been able to overcome some of the apparent drawbacks of SDS-PAGE,1 but it also matches, and in some instances, surpasses techniques such as high performance liquid chromatography (HPLC) in resolution and reproducibility.